BIPN 100 Lecture Notes - Lecture 2: Facilitated Diffusion, Resting Potential, Antiporter

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Mechanism of the sodium/potassium pump (na+/k+ atpase: 3 na+ from the icf (inside) bind to high-affinity sites, atpase is phosphorylated with p(i) from atp. Protein changes conformation: na-binding sites lose their affinity for na+ and release 3na+ into ecf (outside) High affinity binding sites for k+ appear: protein changes conformation. 2 k+ from ecf (outside) bind to high-affinity sites: high-affinity for na+ appear. K-binding sites lose their affinity for k+ and release 2k+ into icf (inside) How are k+ channels gated: 3 different physical mechanisms, s6 gate, ball and chain, selectivity filter gate. Resting cell membranes are most permeable to ____: k+ Facilitated diffusion: carrier proteins: water filled pocket, binding changes conformation, molecules move down [] gradient, does not require atp. Sugar transporters: transporters have specificity and can saturate. Active transport: moves molecules against the gradient. Pumps 2 k+ in: antiporter, maintains the gradient. Symporter: uses potential energy from [] gradient, na+ brings in the glucose molecule.

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