BME 80H Lecture Notes - Lecture 10: Thymine, Kinetochore, Uracil

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Lecture #10- DNA and Chromosome Structure (ch 8 p. 179-189/176-187)
I. Nucleic Acid (DNA and RNA) Structure
A. Molecules and Chemical Bonds
1. Molecules are made up of atoms
Main Atoms = C, H, O, N
( make up >90% of atoms in living systems)
Electronegativity- The affinity for electrons
C & H Have a low electronegativity
O & N have a high electronegativity
2. Atoms are held together in molecules via covalent bonds. (figure 8.4a)
Covalent bonds are strong bonds
Depicted as solid lines
Are formed through the sharing of electrons between 2
atom’s (2e-/bond)
3. Hydrogen bonds form weak, transient interactions between
atoms with partial, opposite charge = dipoles (figure 8.4b).
Dipole = partial charge
B. Nucleic Acids are made up of nucleotides
1. Two kinds of nucleic acids
RNA = Ribonucleic acid
DNA = Deoxyribonucleic acid
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2. Components of Nucleotides (figures 8.5, 8.6)
Nitrogenous base
Purines - adenine (A) guanine (G)
Pyrimidines - cytosine ( C ) Thymine (T, DNA) Uracil (U, RNA)
Sugar
Ribose in RNA
Dexoyribose in DNA
3’ hydroxyl in both DNA and RNA
Phosphates
Bound to 5’C in the sugar
Can be present in 1 (Mono, M), 2 (di, D), or 3 (tri, T) copies
Depicted according to the base/sugar + Phosphates. dGMP is an example
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3. Differences between DNA and RNA (figure 8.5, 8.7)
- DNA Bases: ACGT
-RNA Bases: ACGU
-Ribose in RNA
-Deoxyribose in DNA
-DNA is double stranded
-RNA is mostly single stranded
4. Chains of nucleotides form a strand of nucleic acid (figure 8.7)
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Document Summary

Lecture #10- dna and chromosome structure (ch 8 p. 179-189/176-187: nucleic acid (dna and rna) structure, molecules and chemical bonds, molecules are made up of atoms. Main atoms = c, h, o, n ( make up >90% of atoms in living systems) Dipole = partial charge: nucleic acids are made up of nucleotides, two kinds of nucleic acids. Dna = deoxyribonucleic acid: components of nucleotides ( gures 8. 5, 8. 6) Pyrimidines - cytosine ( c ) thymine (t, dna) uracil (u, rna) Can be present in 1 (mono, m), 2 (di, d), or 3 (tri, t) copies. Depicted according to the base/sugar + phosphates. dgmp is an example: differences between dna and rna ( gure 8. 5, 8. 7) Rna is mostly single stranded: chains of nucleotides form a strand of nucleic acid ( gure 8. 7, the dna double helix (watson-crick) Two strands of dna come together to form the double helix ( gure 8. 9, 8. 10).

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