MCB 2210 Lecture Notes - Lecture 2: Plasmid, Short Hairpin Rna, Small Interfering Rna
3 Apr 2016
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Epifluorescence microscopy: improves contrast and allows specific structures to be labeled: shine a light from outside onto specimens using mirrors and filters. Filters only let certain wavelengths of light through. Only see light emitted by specimen increases the contrast: fluorescence: a molecule absorbs light of one wavelength and them re-emits it as a longer wavelength. Absorbs a higher energy wavelength and emits a lower energy one: fluorophores (fluors): bind to and label specific structures that they are targeted at. There are a variety of different types. What you see in the image is what the fluor targets directly. Antibodies can be used because they bind to specific proteins. Monoclonal antibodies: made by cloning a single b-cell (antibody producer) that recognizes target epitope: all molecules produced are identical. Polyclonal antibodies: mix of different antibodies produced by host animal"s immune response against various epitopes of the target protein: isolated from blood.
