MIMG 102 Lecture Notes - Lecture 25: Nuclear Localization Sequence, Response Element, Intron

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 102
• Professor: Dasgupta Asim

Document Summary

Hiv ctd: hiv latency driven by transcription inactivation & tat deregulation. Some cells long-lived and hard for immune system to get to -> once infected, life-long virus: hiv doesn"t have a transcriptional cascade. Transcriptional cascade -> temporal regulation: make enzymes for replication first before package proteins. Hiv does it differently; makes different species of the genome: multiple rna species. Make genomic rna: full length rna, codes for entire genome. Used to express gag, pol that are cleaved later. Cells don"t like unspliced mrna in cytoplasm; mechanisms for getting unspliced mrna out of the cytoplasm; sometimes packaged as getting spliced. Need to figure out how to get unspliced (genomic rna) & partially spliced mrna to cytosol. Rre (rev response element): included in all unspliced & partially spliced mrna (fully spliced doesn"t have it: rev is essential for nuclear export of intron-containing rna fully spliced -> go to cytosol & make proteins.