MIMG 102 Lecture Notes - Lecture 10: Internal Ribosome Entry Site, Vpg, Picornavirus

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 102
• Professor: Dasgupta Asim

Document Summary

Lecture 10 picornavirus ctd picornavirus mrna review: protein vpg covalently attached to 5" end to nucleotide u -> phosphodiester bond, 5" utr (untranslated region) full of secondary structures. No cap, but still recruits eif4a & 4b to unwind secondary structures -> ribosome binds internally. Secondary structures recruit ribosome: ires (internal ribosome entry site) Makes tons of protein when fused with sequence & put in cell: genome encodes polyprotein: 1st third of genome encodes for p1, 2nd for p2, 3rd for p3. P2 & p3 processed into functional proteins (protease & rna synthesis) 2b is lysis protein -> mutating 2b > cell not lysed. Cut twice in between by specific protease: 2a & 3c: cap-independent translation of poliovirus rna. Can clone each loop separately & see what protein binds -> ribosome recognizes loop-binding protein instead of loop itself. All 4 proteins are host proteins found in nucleus. Patients with lupus (autoimmune disease) make antibodies against la.