MIMG 101 Lecture Notes - Lecture 10: Hypothesis, Root Hair, Lipopolysaccharide

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Fish: hypothesize that the bacteria are essential for growth of the gypsy moth. Use an antibiotic to get rid of the bacteria. When using the antibiotic you should see no growth of the endosymbiont: you hypothesize that e. intermedius degrades cellulose consumed by the gypsy moths into glucose which is then utilized as a carbon source by the gypsy moth. Indicated one test you would perform to test this hypothesis. Be sure to indicate the control and expected results. You could use a radioactively labeled carbon (cellulose) and track the degradation of it and uptake by the moth. You suspect a defect in inducing root hair curling, but when you test for this, you find your mutant is able to induce root hair curling. There could be a defection in the formation of the infection thread (bacteria cant react).

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