LIFESCI 7A Lecture Notes - Lecture 11: Southern Blot, Sticky And Blunt Ends, Plasmid
Document Summary
Restriction enzymes cleave dna at particular short sequences. Dna can be cut at speci c sites, which allows pieces to. Determine whether speci c sequences are present in a segment of. Dna (since cutting dna depends on speci c dna sequences) Cutting dna--use restriction enzymes that recognize speci c short nucleotide sequences in double-stranded dna and cleaves the dna here. The sites that they cleave are restriction sites (~4-6 bp) The ecori restriction site is palindromic and typical of restriction sites. Some have overhangs, some cleave in the middle of restriction site, some have no overhang. Roman letters=the restriction enzyme isolated from that species. Speci city of restriction enzymes ensures that dna in an organism yields the same set of fragments. And any sequences present in cells is contained in a fragment of the same size. Fragments can be visualized in a gel, and extracted (most genomes are too large to do this tho)
