LIFESCI 4 Lecture Notes - Lecture 11: Mutagen, Wild Type, Bacteriophage

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22 May 2018

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Video 12

Mutagen

• UV LIGHT – Crosslinks DNA-thymine dimers

• BASE ANALOGS – substitutions (transitions and transversions)

• ACRIDINE DYES – frameshift mutations (addition or deletion of a base)

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WILD TYPE:

- Anything that grows on E coli K

- Anything that grows on E coli B with small plaque

pattern:

• 1 +: rII-

• 1 -: rII-

• 2 +: rII-

• 1-, 1+: rII+

• +++ or ---: rII+

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