LIFESCI 23L Lecture Notes - Lecture 2: Polyacrylamide Gel Electrophoresis, Serial Dilution, Pipette
Lecture B: Epidemiology
● OVERVIEW OF SKILLS/CONCEPTS
○ 1st part of lab: will perform and analyze disease transmission simulation using a common
bacteria and introduced to proper streaking techniques
○ 2nd part of lab: introduce standard lab techniques designed to prepare you for upper div lab
courses → using micropipetter
○ Skills: agar plate, micropetting, loading SDS page gels, agarose gels, 96 well plates
● BACKGROUND
○ AIDS, SARS, swine flu (H1N1) all caused by viruses
○ Epidemiology=field of science which tries to understand the spread of a disease and
furthermore by predicting its patterns to prevent its occurence
■ Observing H1N1 as pandemic
■ Pandemic model: takes into account infection, recovery, and transport rates of disease
● Places with more back and forth air travel were considered “closer” regarding to
spread of disease of pathogens; simplifies propogation of pathogens into a
simple wave
○ allows prediction of location of origin of pathogen but does not determine
patient zero
■ Patient zero= index/primary case = initial patient in population of
an epidemiological investigation, term first used in studying HIV
■
● LAB DETAILS
○ Explore disease transmission simulation and create an interconnected map
○ 4 rounds of handshakes w/ peers and swab hands/streak on agar gel to observe transmitted
bacteria culture
○ Serial dilution exercise: start w/ unknown solution, make ½, ¼, and ⅛ dilutions
and read ⅛ dilution w/ spectrometer
○ loading/aspiring samples
■ Vertical poly-acryl-amide gel
● Gell has longer and shorter glas plate, with the gel sandwiched
between them
● Gel must be removed to be analyzed
● Pockets not easily visible, appear more like a shadow
● Two gels are assembled in one gel-rig with smaller glass plates
facing each other
■ Horizontal agar gel
● AGAR GEL: solid block of gel with pockets left by a comb;
○ Gel is submerged in buffer and the sample should go into one of these
pockets
■ Submerge pipette tip just above gel and hover over the pocket
■ Push to first stop of resistance, hold plunger, pull out, and release
plunger
■ 96-well plate
● To insert sample into well, touch tip to side of tube and push pipette, allow
sample to run down side of well
● To retrieve sample from well, push down to first stop, find the bottom of the well,
and release push button slowly
○ Lab safety
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
1st part of lab: will perform and analyze disease transmission simulation using a common bacteria and introduced to proper streaking techniques. 2nd part of lab: introduce standard lab techniques designed to prepare you for upper div lab courses using micropipetter. Skills: agar plate, micropetting, loading sds page gels, agarose gels, 96 well plates. Aids, sars, swine flu (h1n1) all caused by viruses. Epidemiology=field of science which tries to understand the spread of a disease and furthermore by predicting its patterns to prevent its occurence. Pandemic model: takes into account infection, recovery, and transport rates of disease. Places with more back and forth air travel were considered closer regarding to spread of disease of pathogens; simplifies propogation of pathogens into a simple wave. Allows prediction of location of origin of pathogen but does not determine patient zero. Patient zero= index/primary case = initial patient in population of an epidemiological investigation, term first used in studying hiv.
