BIS 102 Lecture Notes - Lecture 6: Hydrolysis, Proline, Stage Name
Document Summary
"average mw of an amino acid residue = 11091m 01 ( 65,00091 moi ) flag11091mg ) = 591 a g. Amino acid analysis used to determine which aa and how many of each aa present in the protein. Glass marble reduces loss of protein due to vaporization protein. Add 6m hcl to serve as source of ht ( catalyst) cannot use enzymes bio enzymes are proteins. Autoclave at 110 c overnight to break all peptide bonds through hydrolysis. S - ht g- ht creates electron sink , greater st on the carbon s s s s s g. sit. H gt also cleaves amines to release n hy t. N dt n hut ( b ) Q et n hut ( 2) uncertainty whether protein contains n or d / q or e. Cannot use better nu- such as oh - becauses creates greater uncertainty. Ion exchange chromatography dynamically binds to negative charge through weak bonds , hydrophobic interactions: ifasoaomsinieanaeatmnarae.
