MICROM 461 Lecture Notes - Lecture 10: Giemsa Stain, Apicomplexan Life Cycle, Toxoplasma Gondii
7 May 2020
School
Department
Course
Professor
Document Summary
Microm 461 lecture 10: toxoplasma and intestinal coccidia. Infects a wide variety of animals: definitive host, cats/other felines, where sexually mature forms develop. Intermediate host: rodents (birds, animals eaten by cats, where asexual replication occurs, dead end host, humans, exposed to the oocysts, infectious form. Tachyzoites in mouse brain tissue (giemsa stain: bradyzoites, seen in cysts, stalemate , chronic form, divides slowly, will see large cyst with hundreds of bradyzoite. Bradyzoites in tissue cyst (pas stain: diagnosis, by tissue/biopsy material. Intestinal coccidia: the species, cryptosporidium (parvum/hominis, cyclospora cayetanensis, cystoisospora belli, cause diarrheal disease, diagnosis of intestinal coccidia, look for oocysts in stool, stain with modified acid fast stain, oocysts will appear pink. Cyclospora cayetanensis: look for pink balls in green background, note that there will be other stained stuff in the background because it is fecal matter. What it looks like immediately after excretion from host.
