BSC 315 Lecture Notes - Lecture 19: Ciprofloxacin, Wild Type, Auxotrophy

• School: University of Alabama
• Department: Biological Sciences
• Course: BSC 315
• Professor: Edwin Stephenson

Document Summary

Prokaryotic gene expression, bacteria and their phenotypes. Microbiology: microbiology: study of microscopic, usually unicellular organisms, bacteria, archaea, some fungi, some algae, viruses, using any cellular host, similar methods are employed to cultivate and study unicellular microorganisms. Diversity of life: eukaryotes, animals, plants, fungi algae, protists, prokaryotes, bacteria, archaea. Bacteria: human body carries ~10^13 living bacterial cells. Almost all benign: earlier widely quoted estimate that there are 10x more bacterial cells than human cells per person is now in dispute. Bacterial phenotypes: colony morphology, bacteria and unicellular eukaryotes are grown in liquid culture, or on agar (semi0solid) plates. Colony morphology: example: rough and smooth streptococus colonies; demonstration that dna is the genetic material. Defect in an anabolic (building up) pathway: auxotrophic mutants are studied by testing growth on defined media. If medium contains necessary nutrients, 1 cell grows into a visible colony in 1-2 days.