BSC 310 Lecture Notes - Lecture 35: Semiconservative Replication, Genome Size, Bacteriophage Ms2

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graysardine594

22 Mar 2016

School

University of Alabama

Department

Biological Sciences

Course

BSC 310

Professor

Julie Olson

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Document Summary

T7 is a relatively small dna virus that infects e. coli. Has an icosahedral head and short tail. Brings its own rna polymerase that only recognizes t7 promoters and enzymes that inhibit host rna polymerase activity (cid:1) Genome replication begins at an origin of replication and proceeds bidirectionally with its own dna polymerase (consisting of 1 phage protein and 1 host protein) (cid:1) Has terminal repeats at both ends of the molecule that are used to form concatemers that are cut by a phage specific endonuclease (genomes are identical) (cid:1) (cid:1) (cid:1) Temperate phage with the ability to replicate via transposition facilitated by transposase (cid:1) Mu named because it generates mutations when it integrates into the host cell chromosome (cid:1) (cid:1) Has an icosahedral head, helical tail, and several tail fibers. Host range is determined by the kind of tail fibers made.

Related Questions

QUESTION 1: The rabies virus primarily affects the nervous system. The specificity that the rabies virus has for neuronal host cells is primarily dictated by __________________________.

A. the helical shape of its viral capsid

B. the type of nucleic acid used for its viral genome (single-stranded, antisense RNA)

C. the spikes that protrude from its viral envelope

D. the segmented nature of its viral genome

QUESTION 2: Which of the following genome types has been observed in viruses? To be marked correct, you'll need to select all true statements, as there may be more than one correct answer.

A. Single-stranded RNA

B. Single-stranded DNA

C. Double-stranded RNA

D. Protein-based

QUESTION 3: Choose the correct statement about viral evolution.

A. RNA and DNA viral genomes evolve at equal rates.

B. Cellular genomes mutate at a faster rate than viral genomes due to their large size and increased chance of replicative mistakes.

C. DNA viruses mutate faster than RNA viruses because thymine is more susceptible to mutation than uracil.

D. RNA viruses mutate faster than DNA viruses due to a lack of proofreading replicative enzymes.

QUESTION 4: Your elderly patient is affected by shingles. After careful observation, you note that the virus responsible for the infection has an icosahedral capsid, is enveloped, and has double-stranded linear DNA as its genetic material. Based on this information, in which of the following viral families would you group this viral pathogen?

QUESTION 5: Which of the following is a key difference between lytic and lysogenic bacteriophage replication cycles?

A. Lysogenic bacteriophages release via host cell lysis, whereas lytic bacteriophages bud off from the host cell membrane.

B. The lytic replication cycle ends with host cell lysis and release of newly formed bacteriophage particles, whereas lysogenic replication leads to prophage formation.

C. Lytic replication requires an uncoating step, whereas in lysogenic replication, the bacteriophage's nucleic acid is directly injected into the host cell.

D. Whereas lytic replication enables the infection of several hosts, lysogenic replication only allows for infection of a single host cell.

QUESTION 6: Which of the following is an unsuitable culture method for an animal virus?

A. Direct inoculation of virus onto blood agar

B. Live animal inoculation

C. Tissue culture method

D. Injection of virus into embryonated eggs

BSC 310 Lecture Notes - Lecture 35: Semiconservative Replication, Genome Size, Bacteriophage Ms2

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