BIO-0013 Lecture Notes - Guanine, Chromatin, Thymine
Document Summary
10 9 12: studied pneumonia bacteria, 2 types: rough and smooth, smooth has polysaccharide capsule that stops the immune system from recognizing it, transformation, something from the smooth transferred to the rough to cause it to become virulent, avery, macleod, mccarty, used protease, rnase, dnase, when dnase is used, no transformation, hershey and chase, hereditary material of bacteriophages, capsid remains outside radioactive precursors, labeled with p32 and s35, radioactive phages infect new bacteria, blender, centrifuge, ask: where are s35 and p32, franklin/wilkins && watson/crick, used xray crystallography to deduce the structure of dna. Rosalind"s diffraction pattern: dna double helix, sugar phosphate backbone faces outwards, nitrogenous bases face inwards, right handed helix, wide major groove, narrow minor groove, base stacking on backbone allows for resonance of the electrons between phosphate groups, chargaff"s rules, before the structure of dna was known, studied ratios of nucleic acids, nucleic acids are composed of nucleotide building blocks, ribose or deoxyribose, pyrimidines (1 ring)
