BIO-0013 Lecture Notes - Lecture 10: G2 Phase, G0 Phase, Sister Chromatids
Document Summary
Scientists photobleached the microtubules to help determine this (+) end is on the kinetochore: mt disassemble at the (+) end in anaphase, depolymerizing at the (+) end, cell cycle regulation, majority of cells sit in the g0 phase. Some may sit in that nondividing state forever: s phase must happen before g2 phase, g2 phase must be before mitosis, identifying mitosis promoting factor(mpf) Took cytoplasm from a cell that was in mitosis and transferred it to a cell stuck in g2: cell starts to enter mitosis early. Also took cytoplasm from an interphase cytoplasm and no change occurred: mpf is a complex of cyclin and cyclin dependent kinase(cdk, mpf is a complex of two proteins(referred to as a cyclin/cdk) The enzyme cdk must be bound to cyclin in order to function: when active, cdk phosphorylates(adds a phosphate group) to substrates, cdk phosphorylates other proteins to start m phase, regulation of phosphorylation by kinases and phosphates.
