BIOL 3096 Lecture 30: 30
5 Apr 2016
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Low temp - microtubules fall apart into tubulin. High temp - (33 degrees c + up) - microtubules assemble. In squid - 1 large axon - can take dividing cells take out spindles -> find tubulin monomers. Treat cell homogenates with cycles of temp you isolate tubulin -> do experiment with spindle. Take a homogenate - chill/centrifuge - tubulin in supernatant with other cytoplasmic material. Warm sample and centrifuge- tubulin polymerizes and ends up in pellet without most of cytoplasm. Maps (microtubule associated proteins) - higher molecular weight. Disrupt cell cycle - early in cancer treatment use these drugs as a way of stopping growth of cancer cells( but also stopped general cell growth) Tubulin - gtpase that does treadmilling since it only polymerizes on 1 end. > if atp gamma s + affinity chromatography to find. It was kinesin - allowed materials to move along the micro tubulin.
