01:694:301 Lecture Notes - Lecture 30: Aminoacyl-Trna, Peptide Synthesis, Chain Termination

• School: Rutgers University
• Department: Molecular Biology and Biochemistry
• Course: 01:694:301
• Professor: F Deis

Document Summary

Mrna sequence dictates sequence of aa that make up proteins trna carries proper aa to ribosomes to build polypeptide chain: amino-acyl trna synthetases are enzymes that link aa and nucleic acids. 30. 2-5) and be familiar with the list of trna features on 889-891: accuracy in protein synthesis relies of recognition of 3 base codons on mrna. Each amino acid is attached to amp (adenylate) before being attached to the 3" end of the proper trna; rxn is catalyzed by aa-trna synthethase. Aa get activated through adenylation amino acid ester aminoacyl-trna: understand (fig. Active site: further proofreading increases accuracy (fig. 30. 10: hard distinctions, like that between valine and isoleucine, require proofreading but distinctive amino acids like tyrosine are easier to recognize and don"t require it. Ef-tu protects ester linkage of aa-trna from hydrolysis. Only when the aa-trna is accepted into the a site, is gtp hydrolyzed.