01:447:380 Lecture Notes - Lecture 14: Rna Polymerase Ii, Consensus Sequence, Rna Polymerase I
Document Summary
Transcription: dna rna: transcribing info in language of nucleic acids, highly regulated, requires, all dna is not transcribed, can be affected by cell type, developmental timing, nutrition, environment, dna template, substrate (rntps, rna polymerase. Makes rna 5" 3": a transcription unit: contains everything we need to transcribe rna (i. e. , dna template, regulatory info, only one strand of dna (template strand) is transcribed. Rna polymerase: will move upstream or downstream. Terminator: near termination site: non-template strand reads like mrna, the same dna strand is not template for all genes. Which dna strand is template for gene a? . Where is the promoter for gene a? . Which dna strand is template for gene b? . Which dna strand is template for gene c? . Initiation: rna polymerase binds to dna at promoter. Does not need primer: consensus sequences. Has 2 consensus sequences: when rna polymerase binds, we need to melt the dna, prokaryotic rna polymerase.
