01:119:100 Lecture Notes - Lecture 11: Potentiometer, Nanometre, Transmission Electron Microscopy

• School: Rutgers University
• Department: Biological Science
• Course: 01:119:100
• Professor: Gregory Transue

Document Summary

Originated in the late 1600"s through robert hooke and anton van leeuwenhoek. In 1665, hooke published a description of a compound microscope (compound: having more than one lens) Over 50 drawings of objects observed using it. Leeuwenhoek ground lenses as a hobby and developed single lens microscopes. In 1674, leeuwenhoek began using single lens microscopes to discover and describe bacteria, protozoa, and animal and plant cells. Light microscopy: most common form of microscopy, minimal resolution of 200nm, resolution: distance between 2 points still distinguishable from one another. Aperture value limits resolution: aperture value maximized by immersion oil, avoids refraction of light by air gap of slide and objective. Visual light has wavelength of 400-700nm: light microscope can achieve resolution of 200nm. Magnification calculated by multiplying objective lenses by eyepiece magnification: compound light microscopes (clm): use a set of lenses, mirrors, and light to magnify objects, can only magnify an object about 1000x.