01:119:115 Lecture Notes - Lecture 5: Actual Size, Archaea, Eukaryote
27 Sep 2016
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Cell structure: smallest unit of biological function. A. 1. carries out all activities associated with life: can do everything or be specialized. B. 1. b) skin cells ameba- big, do everything: all share common features and evolutionary history. Microscopy: didn"t know cells existed until invention of microscope. A. 1. a) improved over time (a. 1. a. 1) understanding of cells improved. A. 2. b) didn"t invent microscope however, documented the existence of cells: parameters of microscopy. Ratio of size of image to actual size of specimen. Measure of clarity (b. 2. a. 1) minimum distance between two objects at which. Function of wavelength of radiation passing through specimen (b. 2. b. 1) longer wavelength = lower (worse) resolution. Making things that can be resolved stand out. Higher contrast (b. 3. b. 1) stains (b. 3. b. 2) optics - different filters of light. Size of image : actual size of specimen. Makes things that can be resolved stand out. At best: (c. 2. c. 1) ~1000x magnification (c. 2. c. 2) ~0. 2 micrometers resolution. Focuses beam of electrons through or off specimen.
