FNR 52600 Lecture Notes - Lecture 8: Microsatellite, Muscle Tissue, Cytosine
28 Oct 2018
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Document Summary
4 base pairs: adenine, guanine, cytosine, thymine. Base pairs help form the ladder region of the tightly coiled double helix structure. Full genome is present in the nucleus of every living cell. Human genome (3 billion base pairs) unraveled would be about 6 feet long. Sequence analysis can yield info about species, population, and individual identification. How dna is used in forensics: species identification, sex identification, population assignment, parental analysis, individual identification. Str: short tandem repeats (unique to each individual) Collection of dna from a scene: muscle tissue samples are best for dna analysis, dna degrades over time. Rapid decay of kidney, spleen, liver, brain, and stomach. Clean gloves and new scalpel with each sample. More tissue is not always better (50-100 mg is ideal for mammals) (100-200 mg for fish because of high water content in the tissues) Hair and feather collection should include intact roots if possible.
