ENTM 12800 Lecture Notes - Lecture 9: Serology, Cytosine, Guanine
10 Jul 2017
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Serology: dna analysis in the context of the law, species id. D. transfer stains from unmovable surfaces with sterile cotton swabs and distilled water. Sample storage: careless or ignorant handling of dna can result in a specimen un t for analysis, most evidence is best preserved dry and cold, reduces bacterial growth. D. inhibits dna degradation: water is the enemy. Sources of dna: blood, sweat, skin, mucus. !2: semen, vaginal or rectal secretions, ear wax, saliva, urine. The cell: basic unit of life, humans: composed 100 trillion cells. 3. thymine: cytosine, coding vs. non-coding, dna material is referred to either as, coding: codes for a gene, none-coding: does something else. A. location of interest on a chromosome or genome: alternative possibilities for a gene or genetic locus. A. look at differences in ampli ed sequence length to determine a pattern of differences. B. two main ways to produce length variations: rflp analysis (dna fingerprints, short tandem repeats.
