BIOL 23000 Lecture Notes - Lecture 4: Unified Atomic Mass Unit, Ionization, Electric Potential
27 Nov 2017
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Document Summary
Mass spectrometry sample, as well as determination of their chemical structures. Identification and measurement of the relative abundance of individual molecules in a. Fragments analyzed to determine masses and arrangement of covalent bonds. Can differentiate compounds that vary 1 atomic mass unit (mass of h) Ionization and fragmentation: ionized by stream of high energy electrons. Deflection: fragments focused into fine beam and accelerated past. Detection and analysis: records presence of each ion based on magnetic field, electromagnet, separation of mass determine mass of ion. Powerful way to identify and sequence protein. Enzyme breaks protein down into peptide fragments. Fragments vaporized: in maldi, protein embedded in crystal matrix is vaporized. In esi, protein solution vaporized by application of an electric potential. Vaporized peptide fragments pass through magnetic field that separates them by. Ions accelerated and then deflected by magnet according to mass. Ion flux (ions per unit time) is detected mass/charge ratio. Each protein has signature in mass spectrometer.
