BIOSC 0350 Lecture 15: Lecture O
15 Dec 2018
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Document Summary
Transgenics in mammals: how you make transgenics varies from species to species, mammals, ectopic insertions gene addition. Inject dna into the pronucleus of eukaryotic eggs often results in random insertions into genome: concatemer=several copies in the same region, manipulate endogenous to get specific sequence to jump into genome. G0 generation integrated and a marker gene (in plasmid: transformants only identified in next g1. Drosophila genomic dna: bacteriophage integrase, lysogenic cycle: phage chromosome integrates into bacterial chromosome. Plasmid with attb site and transgene: advantages. Transform agrobacterium with plasmids and spray transformed bacteria on plants. Genomic dna vir proteins: grow embryos from single cells + herbicide to select for transformant herbicider gene. Transgenics are used in many ways to study gene function: rescue mutants with wt copy of gene, confirm which gene is defective in a mutant, ex. T-dna transferred to plant cell and integrates into plant genome.
