BIOSC 0160 Lecture 12: Sanger Sequencing
22 Feb 2016
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Document Summary
Sanger sequencing - based on modification of structural components of dna. Normal nucleotides have oh- group ddntp does not have oxygen so there is not a hydroxyl group. Gel electrophoresis - separates dna fragments based on charge (negatively charged dna will travel towards positive charge) and size (shorter fragments travel via agarose matrix faster than longer dna fragments) X-gal - organic compound consisting of galactose linked to substituted indole; serves as a substrate for beta galactosidase when cleaved, the by product is of blue color. Blue color on plate indicates functional beta galactosidase and nonsuccessful insertion of. White colonies indicate clones with successfully cloned 165 rdna insert. Cells of the white colonies cannot utilize x-gal because the lacz. Genomics - study of genomes (what makes humans the way they are) Transcriptomics - what genes are expressed (certain diseases related to gene expression) Proteomics - study of proteins made by cells under particular conditions.
