BIOSC 0160 Lecture Notes - The Double Helix, Dna Polymerase I, Okazaki Fragments

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Each has a backbone of sugar and phosphate. Each sugar has a nitrogenous base attached to it: a, adenine (purine, c, cytosine (pyrimidine, g, guanine (purine, t, thymine (pyrimidine) 3" end with free hydroxyl on ribose: phosphodiester bond forms between phosphate and hydroxyl, always synthesized 5" 3". 2/6: strands are parallel but run in opposite directions, replication, copying dna, uses enzymes called dna polymerases, begins at the origin of replication. There are several along the length of the dna strand. At each one, strands separate and yield two replication forks. One bubble, to replication forks opposite directions. 5": replication cont, complementarity of strands is key to accurate replication. Semiconservative model: each original strand acts as a template for the new strand, meselson-stahl experiment, hypotheses: Nucleic acids are always synthesized 3" 5". Thus, template is always read 3" 5". Requires rna primers, which are inserted to temporarily create the double strand construct.

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