BIOL 2301 Lecture Notes - Lecture 2: Guanine, Lysogen, Sybr Green I
1 Jun 2016
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A chromosome spread illustrating the human karyotype of 46 chromosomes. Gemisa staining gives characteristic bands on each chromosome and allows for chromosome identification. A karyotype, like the one shown, is prepared from ______ cells. B: either a or b, doesn"t matter, none of the above. Chromosomes can be identified by chromosome painting with specific probes. Repetitive dna sequences have been identified on each chromosome. Dna complementary to these sequences is synthesized and labeled with a specific dye. Probes for each chromosome are labeled with a specific color dye. Probes are allowed to hybridize to (stick to) a prep of metaphase chromosomes. A microscope is used to take a photo of the labeled chromosomes. Dosage compensation: adjusts for differences in the number of sex chromosomes. In many mammals, dosage compensation is accomplished by x inactivation in females. The inactivated x is called a barr body.
