BMCB 658 Lecture Notes - Lecture 18: Rotenone, Antimycin A, Coenzyme Q10

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peachprairie-dog751

19 Aug 2016

School

UNH

Department

Biochemistry, Molecular & Cellular Biology

Course

BMCB 658

Professor

Carrie A.May

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Document Summary

Glycolysis: takes place in cytosol: net: 2 atp plus 2 nadh. Citric acid cycle: takes place in mitochondrial matrix: net: 1 gtp, 4 nadh plus 1 fadh2 (in one turn of tcac) Electron transfer & oxidative phosphorylation: within inner mitochondrial membrane, energy released used to form atp. Difference in [h+] across inner membrane: difference = proton gradient; stored potential energy. Nadh: passes electrons to complex i to coq. Fadh2: passes electrons to complex ii to coq. All electrons: from coq to complex iii cytochrome c complex iv o2. S of cysteine residues forms disulfide bond with inorganic s. Complete reduction requires 2 electrons and 2 h+ Cyt c shuttles electrons from iii to iv. Cyt c passes electrons to cyt a which is part of complex iv. General features in slide 9 of l18. Which complexes drive atp formation, h+ pumping. Coq is soluble in lipid region of membrane. Cytochrome c is loosely bound to inter-membrane face.

Related Questions

1. Compared to the generation of ATP by oxidative phosphorylation, what is a major difference in mechanism of ATP generation by substrate-level phosphorylation in glycolysis?

A. A proton gradient is not involved

B. The ATP produced has less energy content because oxygen was not involved.

C. Substrate level phosphorylation occurs in the inter-mitochondrial space

D. One can't generalize without knowing the substrate.

2. Which of the following statements is true?

A. Because the electrons in NADH are at a higher energy than the electrons in reduced ubiquinone, the NADH dehydrogenase complex can pump more protons than can the cytochrome b-c1 complex.

B.The pH in the mitochondrial matrix is higher than the pH in the intermembrane space.

C. The proton concentration gradient and the membrane potential across the inner mitochondrial membrane tend to work against each other in driving protons from the intermembrane space into the matrix.

D. The difference in proton concentration across the inner mitochondrial membrane has a much larger effect than the membrane potential on the total proton-motive force.

Which of the following accurately describe difference(s) between oxidative phosphorylation and substrate-level phosphorylation during aerobic respiration in eukaryotes?

	a.	Oxidative phosphorylation relies on energy initially derived from redox reactions to power ATP synthase, and substrate-level phosphorylation relies on enzymatic reactions to phosphorylate ADP.
	b.	Oxidative phosphorylation takes place in the inner mitochondrial membrane and substrate-level phosphorylation only occurs in the cytosol.
	c.	Oxidative phosphorylation uses oxygen as a substrate and substrate-level phosphorylation uses phosphate.
	d.	Oxidative phosphorylation takes place during glycolysis and the electron transport chain and substrate-level phosphorylation occurs during the citric acid cycle and chemiosmosis.
	e.	Oxidative phosphorylation produces less ATP than substrate-level phosphorylation.

19. Which of the following is a component of Succinate dehydrogenase in Electron transport chain?

A. Niacin

B. FMN

C. FAD

D. Coenzyme Q

E. Lipoic acid

20. In electron transport the flow of electrons:

A. is bidirectional

B. goes from a high energy state to a low energy state

C. takes place in the outer mitochondrial membrane

D. are used to pump H+ into the mitochondrial matrix

21. Which of the following statements about the electron transport chain is NOT correct

A. It is located in the inner mitochondrion membrane

B. Cytochrome c accepts electrons from complex II

C. Cytochrome c oxidase (complex IV) accepts electrons from Cytochrome c

D. Complex I is called NADH dehydrogenase

E. Coenzyme Q accepts electrons from complex I and complex II

22. In the electron transport chain, the enzyme cytochrome C oxidase:

A. Uses H2O as a substrate

B. Produces HOOH as a product

C. Cannot function if oxygen is absent

D. Accepts electrons from hydrogen ions

E. Uses ADP and Pi as substrates

BMCB 658 Lecture Notes - Lecture 18: Rotenone, Antimycin A, Coenzyme Q10

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