BIO_SC 1200 Lecture Notes - Lecture 1: Tissue Culture, Restriction Enzyme, Plasmid
15 Jan 2015
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What are gmos: organisms that have foreign genes inserted into their dna. Because of this, they will make a new protein that will have a new function (like glowing in the dark) How are gmos made: recombinant dna technology. Isolate gene to make protein: use restriction enzymes proteins made by bacteria that bind to a specific dna sequence. It then cuts the dna at that sequence. Fuse dna and plasmid: recombinant dna. Transform agrobacteria (or e. coli) with recombinant dna: bacteria now has the gene we want to use, transform insert foreign dna into the cell, use transformed bacteria to infect plant, which transforms the plant, gene gun. Isolated gene are used to coat gold particles. Particles are shot into the tissue: most don"t get where they need to go, some make it to nuclei, can grow modified tissue in a tissue culture. Tissue culture regenerates plant: the plant will. Why are gmos made: first gmos were bacteria.
