BIOL 2051 Lecture Notes - Lecture 2: Chlamydomonas Reinhardtii, D-Value (Microbiology), Exponential Growth
Microbial Growth
Growth – increase in the number of cells
Prokaryotes
o Intercalary growth
Binary fission
o Polar growth
Simple budding
Budding by hyphae
Cell division in stalked bacteria
Polar growth without differentiation of cell size
Growth of most microorganisms occurs by the process of binary fission
Cell elongation septum formation completion of septum; formation of walls; cell separation
For Cell Division:
1. DNA replication
2. Formation of divisome
3. Cell elongation
Replication Comparison
In bacteria, like E. coli, the type II topoisomerase involved in relaxing the DNA helix is named DNA
gyrase.
1. DNA Replication
find more resources at oneclass.com
find more resources at oneclass.com
Blue arrows indicate direction of synthesis of new strand on each parent strand
The rate of elongation is about 500 nucleotides per second in bacteria and 50 per second in human
cells
find more resources at oneclass.com
find more resources at oneclass.com
Removing RNA primer and inserting
deoxyribonucleotides
Sealing two fragments on the lagging strand
Replication of Circular DNA
Topoisomerase IV is responsible for unlinking the replicated circular chromosomes
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Growth increase in the number of cells. Polar growth without differentiation of cell size. Growth of most microorganisms occurs by the process of binary fission. Cell elongation septum formation completion of septum; formation of walls; cell separation. For cell division: dna replication, formation of divisome, cell elongation. In bacteria, like e. coli, the type ii topoisomerase involved in relaxing the dna helix is named dna gyrase: dna replication. Blue arrows indicate direction of synthesis of new strand on each parent strand. The rate of elongation is about 500 nucleotides per second in bacteria and 50 per second in human cells. Topoisomerase iv is responsible for unlinking the replicated circular chromosomes. Circular chromosomes have ter sites across from the origin of replication are recognized by tus proteins that block progress of the replication fork: ter sites are sequences in the dna where the tus proteins bind.