DANCEST 805 Lecture Notes - Lecture 17: Scatter Plot, Fluorophore, Nanometre
Document Summary
Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. Flow cytometers are used in a range of applications from immunophenotyping, to ploidy analysis, to cell counting and gfp expression analysis. The flow cytometer performs this analysis by passing thousands of cells per second through a laser beam and capturing the light that emerges from each cell as it passes through. The data gathered can be analyzed statistically by flow cytometry software to report cellular characteristics such as size, complexity, phenotype, and health. In this tutorial, we will look at how a flow cytometer works, how scattered light and fluorescence are detected by a flow cytometer, and how the resulting data can be analyzed. This view shows the primary systems of the flow cytometer schematically. The interrogation point is the heart of the system. This is where the laser and the sample intersect and the optics collect the resulting scatter and fluorescence.
