ACCTG 1 Lecture Notes - Lecture 2: Electron Affinity, Electron Capture Detector, Phenyl Group
Document Summary
The column: where the separation happens, most separations are highly temperature-dependant, so the column is placed in a well-controlled oven, the sample vapor is directed into a column by a carrier gas. Compounds selectively partition between sp (coating) and mp (carrier gas): the oven temperature may be ramped to elute all compounds. Isothermal: temperature stays the same throughout the whole analysis (e. g. 150oc: compounds that are less volatile will take much longer to go through the column in this case; If there are two compounds with similar boiling points, we should go for an isothermal run; The detector: the gas stream from the column, which contains the separated compounds, passes through a detector. Detects compounds with thermal conductivity that differs from carrier gas. Detects compounds that burn or ionize in a flame. Mass selective detector (when combined w/ gc, it is the most powerful identification tool available)
