BIOL 150 Lecture Notes - Lecture 28: Arthur Kornberg, Replisome, Dna Replication
Document Summary
In eukaryotes, dna replication is slower (about 3000 bp/minute) as the cell must remove histones from the dna to allow replication. Some enzymes of dna replication were discovered by arthur kornberg, including the. One strand is replicated continuously, adding bases towards the fork. The other strand is replicated discontinuously, with the new bases being added away from the fork,. Dna polymerase must add bases to the 3" end of a strand that already exists. For this reason, another enzyme, primase, must start the new strand to provide a primer with a 3" end to which the dna bases are added. On the continuous (leading) strand, a single primer is added, followed by dna polymerized following the replication fork (with helicase and topoisomerase) as far as it will go. On the discontinuous (lagging) strand, the new dna is added to a primer until the dna strand reaches the previous primer which is farther away from the replication fork.
