MICRO 302 Lecture Notes - Lecture 2: Antonie Van Leeuwenhoek, Francesco Redi, Sergei Winogradsky

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Explain the controversy surrounding the concept of spontaneous generation and the experiment that resolved the controversy. The theory was disproven when francesco redi used a molding meat experiment to show that maggots arose from fly eggs and did not just appear out of nowhere. Diagram the nitrogen cycle and describe the role of microbes in this cycle. Design a process to isolate nitrogen fixing bacteria. The nitrogen cycle goes from ammonium to nitrites to nitrates. Denitrifying bacteria aid in this process and nitrogen can be fixed by microbes surrounding the legume roots of plants. He developed the winogradsky"s column (a simple device used to culture multiple microbes) and was the one who discovered the role of nitrogen fixing bacteria. Explain the observations that lead up to the acceptance of germ theory and koch"s postulates. Must be able to be grown in pure culture: re-inoculated into healthy host 4. Must be same pathogen and disease as before.

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