MICRO 302 Lecture Notes - Lecture 9: Dna Microarray, Sonochemistry, Dna Replication
28 Jun 2017
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Compare and contrast different methods used to disrupt cells and study cytoplasmic contents. There are 2 major groups of methods including mechanical and nonmechanical methods. Label key structures found in a nucleoid region and explain how dna is supercoiled in a bacterial cell. the nucleoid is the largest structure in a bacterial cell. The supercoiled dna is just positioned randomly within the nucleoid. Plasmids can exist but they are not attached to the supercoiled. Diagram the central dogma of genetics, explaining the role of transcription and translation on protein synthesis. Protein synthesis occurs within the ribosomes and has 3 steps: initiation, translation and termination. mrna gets translated and initiation begins at the ribosomes. trna continues to add amino acids t the chain until a protein is constructed. The protein is then cut from the ribosome and folded according to its function. Explain how transertion alters the shape of the nucleoid region.
