BIOL 2310 Lecture Notes - Gram Staining, Single-Access Key, Aesculin
Document Summary
Examining characteristics on agar plate by the way the bacteria grows (cell morphology), biochemical test, and examining in a microscope: a. The characteristics determined are whether or not it is gram-positive/gram-negative and shape (cocci or rod shaped). In the experiment, mannitol positive/negative, oxidase positive/negative, glucose fermentation positive/negative, lactose fermentation positive/negative, catalase, motility, h2s production, and bile esculin should also be determined. Describe how biochemical test and the dichotomous key will be used to identify bacteria. Biochemical tests include: catalase testing- determining whether bacteria are catalase positive/negative by scraping the bacteria onto a slide and seeing whether or not bubbles form when hydrogen peroxide is placed onto it. Oxidase testing- identifies bacteria by the presence of cco (cytochrome c oxidase). If it is present, it will oxidize the bacteria turning it purple. Substrate utilization testing- identifies bacteria by the use of different substrates and the different colors they change the bacteria to which provides a pattern.
