FRSC 302 Lecture 7: FRSC 302 microscopy notes week 7
10 Feb 2017
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Document Summary
Low power microscopes and stereomicroscopes are used as an optical aid to examine large objects (i. e. bullets rather than specimens mounted on a slide. Objective objective lenses magnification in the range of 2x-10x. Portions of a microscope that (i. e. oculars and objectives) increase the magnification have magnification power engraved on them: total magnification = ocular x objective. Factors to consider when selecting the magnification: fill the view area and minimize white space (achieved under higher magnification) Increasing the depth of field increases the sharpness of the image: working distance distance between objective lens and sample. Resolution is the minimum distance two objects can be separated and still be seen as two objects. High power microscopes or compound microscopes are used as an optical aid to examine small objects (i. e. fibers and hair that are mounted on a slide) A second lens is placed in line with the first lens, this allows for greater magnification.
