BIOL 115 Lecture Notes - Lecture 6: Transmission Electron Microscopy, Scanning Electron Microscope, Lipid Bilayer

18 views2 pages

ochrehornet578

21 Jun 2020

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

All living things are made up of cells. New cells arise only from pre-existing cells. Cells are small because of their surface-area-to-volume ratio: Smaller cells have a larger amount of surface area compared to the volume. An increase in surface areas allows for more nutrients to pass into the cell and more wastes to exit the cell. There is a limit to how large a cell can be while remaining efficient and metabolically active. Resolution of the image varies among different types of microscopes. Compound light microscope: lower magnification than other microscopes, uses glass lenses and light beams to view images, can view live specimens. Transmission electron microscope: 2-d image, uses a stream of electrons to view magnified images, the human eye cannot see the image; it must be projected onto a screen, high magnification, no live specimens. Scanning electron microscope: 3-d image, uses a beam of electrons to view surface structures of specimens, high magnification, no live specimens.

Related Questions

What electron or light microscopy technique would you use toobserve the following?

a) the fine detail of the outer surface of hair cells in theinner ear.

b) the interior and exterior of surfaces of the plasma membraneto support the Fluid-Mosaic Model of membrane structure.

c) very thin cross sections made in Epon in order to visualizethe fine structure of cellular organelles and cell architecture inthe cell interior.

d) particulate matter (like large multisubunit enzyme complexesor viruses) so as to exhibit the subunit structure. Negativestaining.

e) specifically localize a molecule like tubulin in a cell usinga light microscope.

f) the highly organized structure at the molecular level of thespindle of a dividing cell using the lightmicroscope.

g) technique using lasers to optically section large specimens;the images may be computer-enhanced.

h) living cells without killing them by adding stain.

i) a three-dimensional view (almost bas-relief view)of chromosomes in a dividing cell.

j) living cells with high resolution and with extraordinarycontrast at extremely low light levels to keep the cells aliveduring the observation.

k) viewing poorly stained specimens by focusing light on thespecimen alone; the specimen stands out brightly against a darkerbackground

ceruleanfox955

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

limecheetah620

QUESTION 1

The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---

	a.	10x
	b.	20x
	c.	100x
	d.	1000x

QUESTION 2

The part of the microscope that focuses the light on an object is the ___________.

	a.	Iris Diaphragm
	b.	Coarse Focus
	c.	Condenser
	d.	Body Tube

QUESTION 3

The polarizing filter in a PLM that is located above the objective is called the _________________.

	a.	Polarizer
	b.	Analyzer
	c.	Abbe condenser
	d.	Iris

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

1 points

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

maroonmuskrat824

BIOL 115 Lecture Notes - Lecture 6: Transmission Electron Microscopy, Scanning Electron Microscope, Lipid Bilayer

Document Summary

Get access

Related Documents

BIOL 115 Chapter Notes - Chapter 1: Intermediate Filament, Cytoplasmic Streaming, Microfilament

BIOL 115 Lecture Notes - Lecture 32: Endoplasmic Reticulum, Nuclear Membrane, Endomembrane System

BIOL 115 Lecture Notes - Lecture 7: Cell Membrane, Endomembrane System, Passive Transport

Related Questions