CHEM 2541 Lecture Notes - Lecture 5: Column Chromatography, Xanthophyll, Fume Hood
24 Dec 2019
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Separation of pigments from green leafy vegetables by column chromatography. Carotene (a carotene) lycopene (a carotene) cryptoxanthin (a xanthophyll) lutein (a xanthophyll) Structures of the major pigments are shown above. Also present are pheophytins a & b: chlorophylls a & b without the mg2+ion (the two nitrogen atoms are instead attached to hydrogens). Using column chromatography you will take advantage of the difference in polarity of the chlorophylls and carotenoids, which affects their adsorptivity (binding ability) to the chromatographic stationary phase, to separate the mixture of them present in a pigment extract. Before coming to lab remember to consult the sds for chemical substances (limit pigment search to chlorophyll and -carotene) As always, avoid direct contact with chemicals/solvents by working in fume hood as much as possible. As always, wear gloves, eye protection and a fully buttoned lab coat. Part 1: extraction of pigments from dried plant leaves. *if asked, you will be assigned either procedure (a) or procedure (b)
