BIOL 142 Lecture Notes - Lecture 18: Lysogenic Cycle, Intracellular Parasite, Lysis

• School: Emory University
• Department: Biology
• Course: BIOL 142
• Professor: Patrick Cafferty

Document Summary

Biol lecture 18, bacterial genetics iii: transformation and transduction, 3/5/18. Conjugation continued: f+ cells are bacterial cells that have a fertility plasmid in addition to their main chromosome. This plasmid contains genes that encode a sex pilus: the pilus will connect the f+ (donor) and f- (recipient) cells. One strand of the f plasmid will move through the pilus and be replicated within the daughter cell. The remaining single strand in the donor will also replicate. Retrieved from echo alp on canvas: https://canvas. emory. edu/courses/38826/external_tools/90: the origin determines what region of dna is transferred first, at the origin, enzymes nick and open the dna. The chromosomal dna that flanks the f factor will move across the pilus. These adjacent genes (below, a) are transferred first. In the above picture, these are genes a and b. Retrieved from echo alp on canvas: https://canvas. emory. edu/courses/38826/external_tools/90: the single strand of dna becomes replicated as it is transferred.