BIOLOGY 3 Lecture Notes - Lecture 9: Restriction Enzyme, Sticky And Blunt Ends, Plasmid
Document Summary
Step 1: remove the gene from the cow chromosome cut dna. Gene is sliced out of cow chromosome by exposing the cow dna to enzymes that. Restriction enzymes: act like highly specific molecular scissors. Cut dna only at specific sequences called palindromes. Many restriction enzymes cut the dna in a staggered pattern, leaving sticky. Unpaired bases form bonds with complementary bases enzymes selected cuts. Genome: information about the entire suite of genes present in a particular on both ends of bgh gene but not inside the gene ends organism. To determine which restriction enzyme cutting sits surround the gene of interest gene of interest. Cutting the dna generates different fragments, only one of which will carry the. Step 2: insert the bgh gene into the bacterial plasmid. Once removed, inserted into a bacterial structure called the plasmid. Plasmid circular piece of dna that normally exists separate from the bacterial chromosome and can replicate independently of the bacterial chromosome.
