BIOMG 1350 Lecture Notes - Lecture 22: African Clawed Frog, Polarity In Embryogenesis, Blastomere
28 Nov 2016
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Quote of the day: my hair be red like a cherry -lil yachty. Cannot isolate mutants (long generation time and large size) but gene knockdowns are possible (antisense oligos etc) Unfertilized amphibian egg has polarity along the animal-vegetal axis. Fertilization causes asymmetry in ventral to dorsal direction. Sequential induction events and morphogen gradients lead to specification of regions. One region of the blastula can induce formation of an entire tadpole. Basically you can make 2 gastrulation sites if you select the right blastopore from the donor and graft it onto the other side of the host. Cytoplasmic determinants at one pole of the egg result in produciton of two signals that combine to speficy the organizer. Animal pole developes into smaller cells, guessing it turns into the animal. Gray crescent appears after fertilization and makrs future dorsal side. Cleavage almost always cuts crescent in half. Animal region is pigmented, vegetal region is white.
