LIFE 210 Lecture Notes - Dinitrophenol, Glutamine, Citric Acid Cycle
Document Summary
Uses g of e- transfer from nadh to o2. G = p or pmf = - ph + = -1. 4 kcal/mol + -3. 2 kcal/mol. Energy from nadh & fadh2 stored as an electrochemical h+ gradient. Before then it was assumed that all atp synthesis was through substrate-level phosphorylation. Mitchell received the nobel prize in chemistry in 1978. Mitochondrial inner membrane vesicles (mims) and atp synthase. Mitochondrian + mild detergent takes off outer membrane. Sonicate breaks off bits of membranes and forms vesicles. Some vesicles are inside out makes important parts accessible (e. g. atp synthase) Nadh & o2 not required if there is another way to form an h+ gradient. Use to show atp synthesis and nadh + o2 use are coupled. Add no adp stop using nadh + o2. When + g of h+ pumping equals g of e- flow from nadh to o2, nadh and o2 use stop. Mim vesicles treated with urea peripheral membrane proteins dissociated.
