GEN-3000 Lecture Notes - Lecture 3: Eukaryotic Dna Replication, Dna Polymerase Iii Holoenzyme, Rna Polymerase Iii

• School: Clemson University
• Department: Genetics
• Course: GEN-3000
• Professor: Kate Tsai

Document Summary

Indirect evidence based on wavelength and frequency of mutations. Dna**: allows viruses to replicate, dna replication, a single-celled zygote contains 6. 4 billion base pairs of dna, if 1 error in 1 million bp = 6400 errors every time cell divides: ba(cid:272)te(cid:396)ia (cid:396)epli(cid:272)ates (cid:1005)(cid:1004)(cid:1004)(cid:1004) (cid:374)u(cid:272)leotides/se(cid:272) (cid:1005) i(cid:374) (cid:1005) (cid:271)illio(cid:374) e(cid:396)(cid:396)o(cid:396), three models for dna replication, semi-conservative is the winner, each new dna molecule has one strand of old. Initiation: bacterial dna replication, circular chromosome has one (few) origin of replication, helicase and ssb proteins bind to strand, wraps up the dna and allows a little bubble to open; usually at rich areas due to less. Initiator proteins bind to oric, opening dsdna hydrogen bonds: unwinding, dna helicase - enzyme that breaks hydrogen bonds b/t base of strands, cannot initiate unwinding! Always right at the fork: bi(cid:374)ds to the laggi(cid:374)g st(cid:396)a(cid:374)d te(cid:373)plate, (cid:373)o(cid:448)es (cid:1009)"-(cid:1007)" (cid:894)(cid:373)o(cid:448)es fo(cid:396)k! (cid:895) ssdna binding proteins- prevent ss regions from snapping back; within the bubble.