BIOL-4670 Lecture Notes - Lecture 4: Monocytosis, Diluent, Hyperlipidemia
Document Summary
Gm-csf primarily regulates granulocyte and monocyte production: lymphoid stem cells b or t cell linages, lymphocyte production influence by marrow or thymus environment and interleukins. Wbc classification: defense type, phagocytes inflammatory. Immunocytes immune: origin site, myeloid or lymphoid, nucleus shape, presence or absence of granules. Immunophenotye using monoclonal antibodies: cd19/20 b or lymph, cd 2. 3 t (cd 4 or 8) or lymph. Granulocyte: maturation, neutrophils, eosinophils and basophils, most numerous marrow precursors short lifespan, regulated by gm-csf colony stimulating factors, marrow 4 stages granules appear, primary/non-specific granules are lysosomes coated by lipid that contains lytic enzymes. Peroxidase and lysozyme: secondary/specific granules, marrow/blood immature band form, blood mature segmented form. Insert picture from slide 14: eosinophils and basophils are not staged. Immature stages (myleo-, meta-, band) are neutrophils: primary granules appear in promyelocyte stage, primary granules are present in mature cells but do not stain after myleocyte stage under normal conditions, function, neutrophils.
