BIOL 0510 Lecture Notes - Lecture 11: Minimum Inhibitory Concentration, Clavulanic Acid, Antibiotics
7 Nov 2016
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Mechanisms of antibiotic resistance: definitions of antimicrobial resistance. Technical: antimicrobial resistance is the ability of microbes to grow in the presence of a chemical (drug) that would normally kill them or limit growth. Clinical: a microorganism is resistant if there is a high likelihood of therapeutic failure upon antibiotic treatment with a clinically relevant dose: how to measure resistance. Zone of inhibition: plate bacteria in a lawn, soak little pieces of paper in the drug and see if the bacteria die around it. Minimum inhibitory concentration (mic): titration experiment where you start with very high concentrations and continuously dilute it until you find a concentration at which you are no longer inhibiting growth. The mic is the last tube where you see no growth. Lower mic = more susceptible: general mechanisms. Changes in efflux or permeability = most common. Pump out or block drug coming in. Change target of antibiotic so it no longer binds.
