BIOL 5060 Lecture Notes - Lecture 10: Restriction Enzyme, Dna Mismatch Repair, Exonuclease
Document Summary
99. 3% of them get a nucleotide: failing to put a nucleotide changes the length of the whole thing, could have used a better oligote for this sequencing. Blast query (what u start w. ) subject (what u find: blastn your dna seq. Dna seq. in dna database: used for your sequence in the database . When you clone something and want to know what you have, you sequence (blast) can lead to hypothesis regarding the function of what you have . 1) exo iii- can be used for a directional deletion. 2) bal31i- bidirectional deletion: with a collection of deletion (deletion series) you can locate regulators of gene expression and make targeted mutations, for coding regions= 1) pcr- can be used to introduce a low level of mutations; uses a purified polymerase that is more likely to make mistakes. 2) electroten red- carries a mutation in an e. coli gene for mismatch repair; defects in specific proteins required for fixing mistakes.
