BIOL 4510 Lecture Notes - Lecture 3: Giemsa Stain, Cancer Cell, Mutation Rate

• School: Boston College
• Department: Biology
• Course: BIOL 4510
• Professor: Danielle Taghian

Document Summary

Lecture 3: down regulates the gene product, bring down gene product of oncogenes, micrornas, reduce the growth rate of cancer, can take down the overexpression of those oncogenes. Types of dna mutations: aberrant chromosomal number (aneuploidy, aberrant chromosomal structure, translocations (inversions, reciprocal, deletions, amplification of chromosome, extra copy of chromosome, loss of entire chromosome. Ca(cid:374)(cid:272)er (cid:272)ell (cid:272)o(cid:374)ti(cid:374)ues to di(cid:448)ide a(cid:374)d li(cid:448)e (cid:271)e(cid:272)ause (cid:272)ells do(cid:374)"t go through apoptosis a(cid:374)d keep telo(cid:373)eres lo(cid:374)g: despite aberrant chromosomal number or structure, it will still survive, normally would cause a cell to go through apoptosis. Giemsa stains: taken up in different bands of chromosome, tighter=darker, banding pattern unique to each chromosome. Chromosome painting: assign fluorescent dye that generates a color that identifies the specific chromosome, you can see if 1 chunk of chromosome has translocated onto another chromosome. Which came first there is a case for both of these: as cancers progress, there is a higher rate of cin.