BIOL2000 Lecture Notes - Lecture 12: Open Reading Frame, Central Dogma Of Molecular Biology, Dnainfo.Com
16 Jan 2017
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Beadle and tatum made mutants and related mutants phenotypes to genetic changes: forward genetics. Test: damage dna in bread mold, isolate strains that needed more nutrients. Spread cells to form colonies, find defect through replication. Each gene contains info to make an enzyme. Test: metapolic pathway for arginine that needed 3 different enzymes. Provided arginine and can grow, arginine deficinet. Used radiation to create mutants and make genetic screen. Sequence of bases in dna is code where different combos of bases specify the 20 aa. Particular streth of dna: gene: specify aa sequence. Information encoded in base sequnece of dna is not directly translated into aa. Act as link between genes, found in eukaryot cells nucleaus and protein manufacture centers in cytoplasm. Mrna: carry info from dna to site of protein synthesis. Rna polymerase: syntehsizes rna according to base sequence. Central dogma: dna codes for rna which codes for proteins. Sequnce of bases specifies sequence of aa in a protein.
