BIOL 4285 Lecture Notes - Lecture 4: Ldl Receptor, Tau Protein, Ubiquitin
Document Summary
Multifactorial diseases: multiplex genes and the environment: If we want to look for the genes that are influencing diabetes for example: We can use the unique pattern to see which one is segregating with meiosis. If we know where this marker is on the genome, then we can say which gene is responsible for this disease: slide 4: 1 cm map based on microsatellites, they said that every one million nucleotide we have a unique nucleotide. About one in a hundred meiosis theres a separation between the genes for example: slide 5, to create a physical map: Break the dna into fragments using restriction exnzyme. Pcr a fragment of the dna incorporated into the yac to select the colonies based on it. Then order the colonies based on sts (not sequenced yet) Align the physical map with the genetic map based on sts: then sequence the ordered yac.
